Situated near the local zoo and owned by the charismatic polar bear Shirokuma, Shirokuma Cafe is a popular spot for animals and humans alike, allowing them to sit back and relax after a hard day of work. Whether it's a cold beverage or the latest item on his menu, Shirokuma finds joy in being able to serve his customers, often striking up conversations about various subjects. Together with the sarcastic Penguin and the clumsy Panda, they form an odd trio who get themselves caught up in all sorts of misadventures with their other friends such as Grizzly, a bar owner, and Sasako, a human who works at the cafe. From dealing with unrequited love, outdoor camping trips, karaoke sessions, and even the secret to brewing delicious coffee, there's always something bound to be happening in Shirokuma Cafe!

Western Restaurant Nekoya is a popular eatery located on a street corner in a Tokyo shopping district. Serving both traditional Japanese fare as well as Western dishes, this eating establishment is popular among Tokyo's residents. But this seemingly ordinary restaurant is also popular with another type of clientele... While the restaurant is thought to be closed on Saturdays, the truth is that on this special day each week, its doors are instead opened to the inhabitants of other worlds. From dragons and elves to fairies and mages, this restaurant has no shortage of strange customers. Nevertheless, the enigmatic chef known only as "Master" will be waiting to serve up their favorite dishes with a kind smile and keep them coming back for many more Saturdays to come.

The Western Restaurant Nekoya is adored by its local Japanese clientele. The eatery's continued success can be attributed to its tasty western and Japanese dishes, as well as its cheerful head chef, "Master" Tenshu. In Japan, the restaurant appears to be closed on Saturdays, but in actuality, it is the one day that Nekoya transcends to the Other World and opens its doors to guests seeking foreign cuisine. From castles to forests, the "Restaurant to Another World"—as its patrons call it—travels far and wide. Nekoya's diverse and bizarre clientele can expect to be greeted by one of two devoted waitresses—the courteous demon waitress Aletta, or the enigmatic and elven Kuro. As customers, both old and new, pile into the establishment with stories of their respective lands, Master and his staff ensure that everyone is in for a scrumptious adventure of a lifetime.

Kokoa Hoto is a positive and energetic girl who becomes friends with anyone in just three seconds. After moving in with the Kafuu family in order to attend high school away from home, she immediately befriends the shy and precocious granddaughter of Rabbit House cafe's founder, Chino Kafuu, who is often seen with the talking rabbit, Tippy, on her head. After beginning to work as a waitress in return for room and board, Kokoa also befriends another part-timer, Rize Tedeza, who has unusual behavior and significant physical capabilities due to her military upbringing; Chiya Ujimatsu, a waitress from a rival cafe who does everything at her own pace; and Sharo Kirima, another waitress at a different cafe who has the air of a noblewoman despite being impoverished. With fluffy silliness and caffeinated fun, Gochuumon wa Usagi Desu ka? is a heartwarming comedy about five young waitresses and their amusing adventures in the town they call home.